Category: Photomicrography

  1. Gene Paint image library

    GenePaint is a digital atlas of gene expression patterns in various tissues and species with strong focus on mouse embryos. The in situ section mages are in amazingly high resolution so you can clearly see which cells express the gene of interest.

  2. Monochrome conversion of colour immunofluorescence or in situ images

    It is easy to convert colour immuno or in situ figures to monochrome whilst emphasizing the positive staining colour and repressing the background stain.

  3. Loading sets of images into powerpoint slides

    Sometimes one may want to generate a PowerPoint slide with a set of images - for example a set of micrograph images to discuss with your colleagues (or a lovely set of your holiday photos to make your friends jealous). You can do this by manually adding each image, one by one, then resizing, repositioning, formatting .... , but there is a much quicker way. Here is a guide to automating the process.

  4. Fluorescence Images: Merging and optimising

    If you are using fluorescence microscopy you may need to merge images taken with different filter sets - for example DAPI to pick out nuclei together with fluorescent staining with or or more specific antibodies. Commonly you will want to merge these images into a composite. Image optimisation and merging can be achieved easily using the free Fiji package with ImageJ.

  5. Improving photomicrographs

    Photomicrographs looking a bit muddy? I have made some notes on a simple way to improve things.

  6. Focus stacking for photomicroscopy

    Focus stacks are a simple way to get sharp photos when your microscope section is not flat. Compare the left thumbnail with out of focus parts with the right, which is sharp all over
    focus stack 1 Focus stack using CombineZP

  7. Improving Your Photomicrography

    Some quick tips to improve photomicrographs.